1State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Chifeng Institute of Agricultural and Animal Sciences,Chifeng 024031,Inner Mongolia,China
Abstract:To clarify the resistance background of pepper germplasms in the Inner Mongolia Autonomous Region,the disease resistance of 98 pepper germplasms from the Inner Mongolia were identified by molecular markers combined with artificial inoculation for pepper mild mottle virus disease(PMMoV),tomato spotted wilt virus disease(TSWV),bacterial spot disease of pepper and anthracnose disease of pepper.The results showed that 6 and 8 pepper germplasms carried the target fragments using by the PMMoV resistance locuslinked markers IH1-04 and 087H3T7,respectively.Eight germplasms were resistant to PMMoV after artificial inoculation,which were consistent with the presence of resistence locus screening by marker 087H3T7.Four
pepper germplasms carried the linkage marker SCAC568 of TSWV resistance gene,Tsw,which was consistent with the identification results of artificial inoculation.Ten pepper germplasms were detected to be positive by the bacterial spot disease-resistance locus Bs2-linked markers Bs2-L1/R1,Bs2-S45 and Bs2-14F/R.No anthracnose resistant germplasms was found with microinjection(wounding)inoculation method using the fungal(Colletotrichum acutatum)isolate of pepper anthracnose.